這項研究被發佈在最近Polytechnic City of Innovation所舉行的研討會上，與會者包括最早開發出這項技術的義大利科學家。
Gianluigi Colalucci是修復西斯汀教堂的主要成員之一，他和同事將過去的專業經驗分享給正在嘗試修復西班牙桑托斯橫斯教堂的工作團隊。Colalucci及同事Carlo Giantomassi已經將這項由義大利微生物學家Giancarlo Ranalli所開發出來的技術應用在修復Campo Santo di Pisa的畫作上。該教堂位在著名的比薩斜塔旁。
因此，微生物學教授Rosa Maria Montes以及生物學家Pilar Bosch一同前往義大利，向原技術開發人員學習如何利用細菌去除義大利畫作上的外來物。
Bacteria 'Trained' to Clean Ancient Artworks
Spanish art experts are employing bacteria to restore priceless 400-year-old frescoes in a church in the southern city of Valencia using a technique developed to restore equally precious frescoes in Italy.
Spanish restorers are testing this new technique on the frescoes of Antonio Palomino from the 17th century in the Church of Santos Juanes in Valencia.
They have shown that a special type of bacteria is capable of cleaning works of art in a fast, specific and respectful way as well as being non-toxic to the restorer or the environment.
The team that completed the tests are from the Institute of Heritage Restoration and the Centre for Advanced Food Microbiology, both from the Polytechnic University of Valencia.
These developments were announced at a recent seminar held at the Polytechnic City of Innovation, which was attended by the Italian creators of these techniques using bacteria.
One of Italy's leading restorers, Gianluigi Colalucci, who restored the Sistine Chapel, and his colleagues have shared their expertise with the Spanish restorers working on the murals of the Church of Santos Juanes.
Colalucci and colleague Carlo Giantomassi had applied the technique, developed by Italian microbiologist Giancarlo Ranalli, in their restoration of the wall paintings of Campo Santo di Pisa, next to the city's famous Leaning Tower.
The microrganism selected by the restorers is part of the Pseudomonas family and it has been converted into a gel for the works.
"The frescoes on which we are using it were damaged after the Second World War," Giantomassi told the seminar participants.
"To remove them from the walls they were using animal glues which in time have become insoluble and impossible to remove with traditional methods. The bacteria are able to eat these glues and leave the painted section intact."
In the past, to remove foreign materials encrusted on ancient artworks restorers used reactive chemicals that are non-selective and toxic. Or they tried to wear away the crust by mechanical means, which is time consuming and can damage the paint.
The Valencia project began when the Institute of Heritage Restoration was working with the murals of the Church of Santos Juanes, which were virtually destroyed after a fire in 1936 and were improperly restored in the 1960s.
The researchers had difficulty dealing with salt efflorescence, the white scabs caused by the buildup of crystallized salts and the enormous amount of gelatine glue remaining on the pulled-off murals.
So microbiology professor Rosa Maria Montes and biologist Pilar Bosch travelled to Italy to learn from the originators about their work using bacteria to remove foreign matter from Italian artworks.
"By the action of gravity and evaporation, the salts of organic matter in decomposition migrate to the paintings and produce a white crust hiding the work of art and sometimes can also cause the loss of the painting layer," says Bosch.
Back in Valencia, the Spanish team say they "trained" the most suitable strain of Pseudomonas bacteria to literally eat the saline efflorescence found in the lunettes of the vault behind which pigeons nest.
The Spanish scientists reduced the application time and created a new way of using the bacteria.
Bosch said, "In Italy, they use cotton wool to apply the micro-organisms. We, however, have developed a gel that acts on the surface, which prevents moisture from penetrating deep into the material and causing new problems."
"After an hour and a half, we remove the gel with the bacteria. The surface is then cleaned and dried. Without a wet environment, the remaining bacteria die," he explained.
The new method has been tested on two lunettes of the vault in the Church of Santos Juanes and scientists expect to apply it on another two.
The Valencia research project was funded through the R&D support program of the Polytechnic University of Valencia and concluded with Bosch's doctoral thesis, which received a grant from the Spanish Ministry of Science and Innovation. The scientists are considering partnerships with private businesses.
"After the good results of the tests, we will continue the studies and improve the technique with the aim of transferring it to other surfaces," said Bosch. "As in nature we find different species of bacteria that feed on almost anything, we are convinced that we can eliminate other substances from different types of materials."